Development of Anti-hepatofibrotic Herbal Drug (CGX)

Predicting In Vivo Anti-Hepatofibrotic Drug Efficacy Based on In Vitro High-Content Analysis

BACKGROUND/AIMS Many anti-fibrotic drugs with high in vitro efficacies fail to produce significant effects in vivo. The aim of this work is to use a statistical approach to design a numerical predictor that correlates better with in vivo outcomes. METHODS High-content analysis (HCA) was performed with 49 drugs on hepatic stellate cells (HSCs) LX-2 stained with 10 fibrotic markers. ~0.3 billio...

Development of anti-malaria herbal preparation / drugs from local medicinal plants

1. The screening for potential anti-malaria properties by bioassay guided fractionations. The biological properties were carried out using the plasmodium lactate dehydrogenase assay (pLDH) (Makler, 1993). 2. The harmful effect of the extract was determined by the MTT assay (Mossman, 1983). 3. The extracts were tested for its efficacy in animal model using rodent malaria parasite, P. berghei usi...

Development of anti-malaria herbal preparation / drugs from local medicinal plants

1. The screening for potential anti-malaria properties by bioassay guided fractionations. The biological properties were carried out using the plasmodium lactate dehydrogenase assay (pLDH) (Makler, 1993). 2. The harmful effect of the extract was determined by the MTT assay (Mossman, 1983). 3. The extracts were tested for its efficacy in animal model using rodent malaria parasite, P. berghei usi...

An herbal formula, CGX, exerts hepatotherapeutic effects on dimethylnitrosamine-induced chronic liver injury model in rats.

AIM To evaluate the therapeutic effect of Chunggan extract (CGX), a modified traditional Chinese hepatotherapeutic herbal, on the dimethylnitrosamine (DMN)-induced chronic liver injury model in rats. METHODS Liver injuries were induced in Wistar rats by injection of DMN (ip, 10 mg/mL per kg) for 3 consecutive days per week for 4 wk. The rats were administered with CGX (po, 100 or 200 mg/kg pe...

Need of Innovative Approach in Herbal Drug Development